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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis
doi: 10.1186/s13287-017-0723-y
Figure Lengend Snippet: Primary and secondary antibodies used for MPC ± PPS cytometric analysis
Article Snippet: IgM FITC ,
Techniques: Purification, Control
Journal: Stem Cell Research & Therapy
Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis
doi: 10.1186/s13287-017-0723-y
Figure Lengend Snippet: Concentration-dependent uptake of PPS-FITC by MPCs (2.5 × 10 5 cells/well) determined using the fluorometric assay. Highest uptake was observed with PPS-FITC concentrations of 5.0 μg/ml which were significantly different to 1.0 and 2.5 μg/ml. a p < 0.004; b p < 0.012. FITC fluorescein isothiocyanate, PPS pentosan polysulfate
Article Snippet: IgM FITC ,
Techniques: Concentration Assay
Journal: Stem Cell Research & Therapy
Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis
doi: 10.1186/s13287-017-0723-y
Figure Lengend Snippet: Fluorescence microscopy images of MPCs (6000/well) cultured with 2.5 μg/ml PPS-FITC for 24 h, fixed in Histochoice MB/ethanol and stained with propidium iodide. a A 2-s exposure image of the MPCs outlined by the low-level background autofluorescence and the higher emission arising from the PPS-FITC located within the cells ( arrows ). b The same field as a , but with 60-s exposure with excitation at 535 nm confirming the presence of PPS-FITC within the nucleus of the MPCs ( arrows ). FITC fluorescein isothiocyanate, PPS pentosan polysulfate
Article Snippet: IgM FITC ,
Techniques: Fluorescence, Microscopy, Cell Culture, Staining